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MI 3-in-1 Immunoassay Testing Principle
Myo/CK-MB/cTnI mycardial infarction (MI) 3-in-1 test panel uses a solid-phase chromatographic immunoassay technology to qualitatively detect the elevation of myoglobin (Myo), CK-MB, and troponin I (cTnI) in human serum or plasma samples. When serum or plasma sample is added to the sample wells, it will move along the three independent membrane strips. Myo, CK-MB and cTnI in the sample, if any, will be bound to specific antibody-dye conjugates and move to the test regions (T) where immobilized anti-myoglobin, anti-CK-MB, and streptavidin have been pre-coated respectively. The cardiac marker-antibody-dye complexes will bind to the corresponding immobilized antibodies or streptavidin in the test regions. Red lines will appear, indicating a positive result. Unbound dye complexes move on to the control region (C) and will be captured there.
The red line in the control region provides a internal control mechanism.
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