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Bird Flu ELISA Test Kits
   
   
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H5N1 Bird Flu ELISA Testing Kit    

Bird Flu ELISA Test Kits

H5N1 ELISA kits-Avian Infuenza Antigen and Antibody Test

Bird Flu ELISA test kits, also known as Bird Flu EIA kits, can be divided into two subcategories according to the chemicals tested: Bird Flu antigen ELISA test kits and Bird Flu antibody ELISA test kits. Avian Influenza Antigen ELISA kits are two-step incubation assay and Avian Influenza antibody ELISA kits are one-step incubation test. Avian influenza Antigen EIA kits can be performed with both human samples and bird body fluid, while Avian influenza Antibody EIA kits Antibody ELISA kits are for Human clinical testing only.

1, H5N1 Bird Flu Antigen ELISA Testing Kits

H5N1 antigen ELISA kits are enzyme-linked immunosorbent assay (ELISA) for qualitative detection of hemagglutinin (HA) of Influenza A Virus and H5 strain in avian excretes, human nasopharyngeal aspirates,swabs, throat swab, nasal wash, bronchial wash, chicken embryo whole virus inoculation or viral lysates, etc.

   
   
H5N1 Avian Influenza Dot ELISA Device    

H5N1 Bird Flu Antigen ELISA screening kits assay principle: the microwells are pre-coated with antibodies (anti-HA"Ab") specific to the hemagglutinin (HA) of the H5 Influenza strain. During the first incubation after sample is added into the microwells, the specific anti-HA"Ab"-HA immunocomplex will form if H5 HA presents, and will be captured on the solid phase. During the second incubation after the unbound proteins being washed away and second H5 HA specific anti-HA"Ab" conjugated to horseradish peroxidase (anti-HA"Ab" HRP) is added, anti-HA"Ab" - HA - anti-HA"Ab"HRP sandwich immunocomplex will form. After specific treatment with the reagents enclosed in H5N1 Bird Flu ELISA Antigen testing kits, the immunocomplex will turn into yellow, indicating a positive result. The amount of the color can be measured and is proportional to the amount of HA in the sample. Wells containing samples negative for H5 HA remain colorless, standing for negative result.

 
       


2, H5N1 Avian Influenza Antibody ELISA Screening Kits

H5N1 Avian influenza antibody ELISA Screening kits are for qualitative detection of antibodies (anti-HA) to hemagglutinin (HA)  of Influenza A viruses, H5 strain in human serum or plasma samples.

H5-HA(Ab) ELISA kit is based on solid phase, one step incubation competitive principle ELISA. H5 Influenza specific, anti hemagglutinin (anti-HA) antibodies, if present in the sample, compete with monoclonal Influenza H5 antibodies conjugated to horseradish peroxidase (anti-HA"mAb"HRP) for a fixed amount immunological Ag-Ab complex that has been pre-coated inside wells. This complex contains viral lysate of H5 HA, and monoclonal H5 specific anti-HA. When no H5 specific anti-HA presents in the sample, anti-HA"mAb"HRP will be bound with the H5 HA inside the wells and any unbound HRP-Conjugate is removed during washing. Chromogen A and B solutions are added into the wells and during incubation, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. No or low color developing suggests the presence of specific antibodies to hemagglutinin of influenza Type A, H5 strain.

  • Influenza A ELISA kits are intended for clinical identification of the H5 strain of influenza A viruses;
  • H5 antigen and antibody ELISA screening kits are suitable for testing large numbers of samples;
  • Bird Flu IVD ELISA Testing Kits are For In-Vitro use and Professional Use only.

Catalog No.

Product Name

Package

Samples Used

Storage Condition

Shelf Time

BFL 951

Bird Flu Antigen ELISA Kits

96 Tests/box

Avian excretes, human nasopharyngeal aspirates,swabs, throat swab, nasal wash, bronchial wash, chicken embryo whole virus inoculation or viral lysates, etc.

2-8℃

12 Months

BFLU 952

Bird Flu Antibody ELISA Kits

96 Tests/box

Human serum or plasma

BFLU 461

H5N1 Dot Antibody ELISA Kits

6 Tests/Box

Human serum or plasma

   
             
           
       
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